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Pak. J. Bot., 45(2): 643-648, 2013.

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ANTIMICROBIAL POTENTIAL OF DIFFERENT SOLVENT EXTRACTS OF TOBACCO (NICOTIANA RUSTICA) AGAINST GRAM NEGATIVE AND POSITIVE BACTERIA

JEHAN BAKHT^1*, AZRA¹ AND MOHAMMAD SHAFI²

Abstract: The present study was aimed to evaluate the antimicrobial activity of tobacco extracts from Nicotiana rustica at different concentration in different polar solvents. For this purpose 6 different extracts were prepared, using 5 different polar solvents viz., ethanol, ethyl acetate, n-hexane, acetone, butanol and water. Four different concentrations (6, 12, 18 and 24 mg Disc^-1) of each extract were subjected for preliminary antibacterial screening against 7 pathogenic bacteria by Kirby-Bauer Disk Diffusion method. The result of In vitro antibacterial screening showed that 6 extracts from Nicotiana rustica had different ranges of antibacterial activities.  The ethyl acetate extracts showed more potent effects followed by butanol, very little in the ethanol extracts while no significant inhibitory effects were observed in acetone or hexane extracts. When tobacco extracts were studied for their antibacterial potential against gram-positive bacteria and gram-negative bacteria, ethyl acetate extracted samples were more effective against Bacillus cereus, Staphylococcus aureus, and Erwinia carotovora  at highest concentrations. Hexane and acetone extracted samples did not inhibit the growth of both gram +ive and –ve bacteria.

¹Institute of Biotechnology and Genetic Engineering, University of Agriculture Peshawar, KPK Pakistan

²Department of Agronomy, University of Agriculture Peshawar, KPK Pakistan

* Corresponding author email: jehanbakht@yahoo.co.uk