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Pak. J. Bot., 45(2): 629-634, 2013.

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SYNERGISTIC EFFECT OF AUXINS AND CYTOKININS ON PROPAGATION OF ARTEMISIA AMYGDALINA (ASTERACEAE), A CRITICALLY ENDANGERED PLANT OF KASHMIR

RAFIA RASOOL^1*, BASHIR A. GANAI¹, AZRA N. KAMILI², SEEMA AKBAR³ AND AKBAR MASOOD¹

Abstract: Efficacy of different auxins and cytokinins was studied on critically endangered and endemic plant Artemisia amygdalina Decne. of Kashmir by exploiting the morphogenetic potential of  shoot tip explants. Four phytohormonal combinations (NAA and BAP; Kn and 2, 4-D; BAP and 2, 4-D; NAA and Kn) were used to find the viable combination for its optimum proliferation and growth. Murashige and Skoog (MS) medium fortified with 2 µM of α- napthaleneacetic acid together with 1 µM of 6-benzyladenine produced up to 19 shoots per shoot tip after 6 weeks under optimal culture conditions. Regenerated shoots showed maximum rooting on MS basal media with an average of 10 roots per explant. Rooted plants were transferred to soil in green house where they exhibited normal growth. This is the first In vitro regeneration report of A. amygdalina which focuses on conservation of its unique germplasm.

Abbreviations: BAP: 6-benzyladenine, 2, 4-D: 2, 4, dichlorophenoxyaceticacid, Kn:  Kinetin, MS:  Murashige and Skoog, NAA: α- napthaleneaceticacid, PGR: Plant growth regulators.

¹Department of Biochemistry, University of Kashmir, Srinagar-190006, Jammu and Kashmir, India

²Plant Tissue Culture Laboratory, Centre of Research for Development, University of Kashmir,

Srinagar-190006, Jammu and Kashmir, India

³Regional Research Institute of Unani Medicine, University of Kashmir Campus,

Srinagar-190006, Jammu and Kashmir, India.

^*Corresponding author e-mail: bbcganai@gmail.com; rasoolrafiya@gmail.com