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Pak. J. Bot., 45(2): 519-523, 2013.

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MOLECULAR ASSESSMENT FOR GENETIC IDENTIFICATION AND STABILITY OF CYMBIDIUM SANDERAE (ORCHIDACEAE)

PORNARONG SIRIPIYASING¹, KOBSUKH KAENRATANA², PIYA MOKKAMUL¹ AND ARUNRAT CHAVEERACH^3*

Abstract: In order to verify a natural hybrid or a wild type species of Cymbidium sanderae, the putative parents and four more species were collected for genetic stability assessment by molecular data. Dendrogram constructed from 13 Inter Simple Sequence Repeat (ISSR) profiles distinguished each species with genetic distance (D) values of different species at similar level. The D values of C. sanderae compared to the expected parents, C. lowianum, C. insigne, and C. eberneum are 0.500, 0.501, and 0.470, respectively. While those compared to other species, C. haematodes, C. ensifolium, C. sinense, and C. munronianum are 0.501, 0.490, 0.496, and 0.485, respectively. Additionally, overall D values between other samples range from 0.414 (C. ensifolium and C. sinense) to 0.547 (C. insigne and C. haematodes). Regarding the D values mentioned, they appear to be at the same or similar levels. Therefore, C. sanderae is a species just as the other studied species are, and this means that it should be classified as a wild type species.

¹Department of Biology, Faculty of Science, Mahasarakham Rajabhat University, Thailand

²Pakkret Floriculture Company, Nonthaburi, Thailand

³Department of Biology, Faculty of Science, Khon Kaen University, Thailand

^*Corresponding author’s e-mail address: raccha@kku.ac.th, Tel:+66 82 3095690