Back to Contents

Pak. J. Bot., 45(2): 513-517, 2013.

FREE FULL TEXT PDF

FAST EXTRACTION OF BIOACTIVE FATTY ACIDS FROM THE PERILLA SEEDS BY SMASH TISSUE EXTRACTION

WEIZHUO TANG^1,4, YANLING SUN¹, YANZE LIU², YINGFENG WEI³ AND YUQING ZHAO^1,4*

Abstract: In this study, smashing tissue extraction (STE) was successfully applied to extract bioactive fatty acids from the Perilla seeds. As control experiment, four conventional extraction methods including leaching, Soxhlet, ultrasonic-assisted, and supercritical-CO₂ fluid extraction have been comparatively investigated. The content of bioactive fatty acids from each extract were quantified with the help of GC-FID in order to further optimize the extraction process of STE. The result shows that STE is an efficient extraction method to obtain fatty acids due to its simple, rapid preconcentration of required components and the highest oil yield among those methods tested. The optimized parameters of STE obtained through orthogonal design were the ratio of liquid to solid material at 8:1, the power of extraction at 50w, and extracting time 30 second.

¹Shenyang Pharmaceutical University, Shenyang 110016, P. R. China

²Bio-Organic and Natural Product Laboratory, MRC 311, McLean Hospital/Harvard Medical School, 115 Mill Street, Belmont, MA 02478, USA

³Liaoning Jiashi Nutritional Plant Oil Development Co.Ltd. Shenyang 110035, P. R. China

⁴Key Laboratory of Structure-Based Drug Design & Discovery (Shenyang Pharmaceutical University), Ministry of Education, Shenyang 110016, P. R. China

^﹡Corresponding author’s e-mail: zyq4885@126.com. Tel/Fax : 86-24-23986521