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Pak. J. Bot., 45(2): 501-505, 2013.

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EFFICIENT REGENERATION OF A POTENTIAL MEDICINAL PLANT OCHRADENUS BACCATUS DELILE FROM COTYLEDON AND SHOOT AXIS

FAHAD AL QURAINY, MOHAMMAD NADEEM^*, SALIM KHAN, SALEH ALANSI AND MOHAMED TARROUM

Abstract: Ochradenus baccatus (Del.) is  one of the high value medicinal plant of Saudi Arabia. The plant is used particularly in lowering  blood cholesterol level and counteracting malaria. The propagation of the species in Saudi Arabia and other allied areas is scarce. Therefore, we have developed an effective regeneration method of mass propagation of this potential plant. In vitro shoot multiplication was obtained from cotyledon and shoot axis on the MS medium containing 2.0 µM (BA) and 4.0 µM (NAA). The shoots were further multiplied on the MS medium containing (0.5-5.0 µM) BA and 2ip individually. BA was better in enhancing the multiplication of shoots. These shoots were routinely cultured on the MS medium with BA at 1.0 µM concentration which resulted in a fair amount of shoots per subculture at one month interval. The multiplied shoots were exposed to the MS medium supplemented with IBA and NAA. This  resulted in reduced percent rooting. Rooting was enhanced when the shoots pulse treated with IBA (25-200 µM) at 5, 10 and 15 days. When the shoots were  transferred to the  MS medium without any PGR  maximum (96.6%) rooting was achieved on treatment with 100.0 µM IBA  for 10 days. Plants were acclimatized on different soils. Highest (70%) survival was obtained on sterilized soil with FYM. Efforts are being made to transplant the In vitro developed plants  to the  natural habitat.

Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia

*Correspondening author’s e-mail: mohammadnadeem911@hotmail.com