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Pak. J. Bot., 44(5): 1593-1599, 2012. |
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Updated: 10-10-12 | ||||
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MICROBIAL ASSISTED PHYTOEXTRACTION OF METALS AND GROWTH OF SOYBEAN (GLYCINE MAX L. MERRILL) ON INDUSTRIAL WASTE WATER CONTAMINATED SOIL
IRFAN ALI1, FAZAL HADI2* AND ASGHARI BANO1
Abstract: Pots experiments were made to investigate the role of effective microorganisms (EM) in improving phytoextraction of metals (Cd+2 and Mn+2) and growth of soybean plant in industrial waste water polluted soil. Waste water applications to soil were made in four different dilutions (i.e. 25%, 50%, 75% and 100%). Effective microorganisms were added into waste water prior to application. Effect of treatments on growth parameters was studied. The Cd+2 and Mn+2 concentrations in different parts of plant were measured by Atomic Absorption Spectrophotometer. Plant height significantly increased at all treatments except at 25% waste water treatment. Plant dry biomass and oil contents in seed significantly increased with all treatments compared to control but were higher at low concentration of waste water. Waste water treatments significantly increased the Cd and Mn accumulation in plant while inoculation of EM further enhanced the metals accumulation. The maximum accumulation of Cd and Mn found in plant treated with 100% waste water in combination with effective microorganisms. At harvest, the Cd+2 concentration decreased in leaves but increased in roots followed by stem > seeds, while, Mn+2 accumulation increased in leaves followed by roots > stem > seeds. Conclusively, EM enhanced the phytoextraction of Cd and Mn and also increased the oil contents in soybean on polluted soil. These findings suggest further investigation to find out a suitable concentration of industrial waste water in combination with EM for better growth of soybean and improving phytoextraction of metals.
1Department of Plant Sciences, Quaid-i-Azam University, Islamabad, Pakistan 2Department of Biotechnology, University of Malakand, Khyber Pakhtunkhwa, Pakistan *Corresponding author’s e-mail: dr.fhadi@uom.edu.pk; +92-3476873779 |
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