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Pak. J. Bot., 44(5): 1527-1532, 2012.

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APPRAISAL OF GENETIC DIVERSITY OF DIFFERENT PEACH CULTIVARS AND  GENOTYPES THROUGH RAPD MARKERS

JEHAN BAKHT^1*, NAZMA JAMAL¹ AND MOHAMMAD SHAFI²

Abstract: The present study was amid to investigate the genetic diversity of twenty peach cultivars and genotypes by RAPD primers at the Institute of Biotechnology and Genetic Engineering, KPK Agricultural University Peshawar. The result indicated that fifteen primers (GLCO9, GLC20, GLA20, GLA13, GLB10, GLB20, GLB06, GLB19, GLA19, GLB19, GLD16, GLB15, GLA15, GLB12, GLB11) gave genetic distance among the peach cultivars and genotypes under study by PCR amplification. Average genetic diversity (estimated as genetic distance) ranged between 12 and 58%. The molecular size of most of the bands were from 150bp to 1000bp. Based on dandrogram analysis, Khyber 1 and Khyber 2 was grouped in cluster A, and Tex-A6-69 and BY-8-135 in cluster B, Candan and 6A were most closely related cultivars and genotypes among the 20 peach cultivars and genotypes while Lering, Flam crest, Tex x-9, early grand and Floradaking were distinctly grouped when compared with the rest of population.

¹Institute of Biotechnology & Genetic Engineering, KPK Agricultural University Peshawar, Pakistan

²Department of Agronomy, KPK Agricultural University Peshawar, Pakistan

*Corresponding author email: jehanbakht@yahoo.co.uk