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Pak. J. Bot., 44(5): 1509-1512, 2012. |
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Updated: 10-10-12 | ||||
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CHARACTERIZATION OF WHEAT GENOTYPES USING RANDOMLY AMPLIFIED POLYMORPHIC DNA MARKERS
KHATIBA BIBI1, INAMULLAH1, HABIB AHMAD1*, SIRAJUD DIN3, FIDA MUHAMMAD2 AND MUHAMMAD SAJJAD IQBAL1
Abstract: Genomic DNA from the genotypes Khyber-87, Saleem 2000, Suleman-96, Pir Sabak-2004, Pir Sabak-2005, NRL-0320, NRL–0517, RAS-II, Tatara, RAS-I, Bathoor, Fakhare Sarhad, Bakhtawar-94, Inqilab-91, Haider-2002, Noshera-96, and Auqab-2000 was amplified using RAPD primers. Loci of 100-1400 bp sizes were amplified and on average 3.51 loci per genotype were amplified. Average genetic diversity using the twelve RAPD primers ranged from 30-90%. Phylogenetic relationship among the wheat genotypes was carried out using dendrogram analysis. The seventeen genotypes were clustered in five groups. It was found that the genotypes Pirsabak 2004 and Ras 1 were most distantly related to Khyber-87. It is suggested that these two genotypes be crossed with Khyber 87 for creating maximum genetic variations.
1Department of Genetics, Hazara University, Mansehra, Pakistan 2Department of Plant Breeding and Genetics, NWFP Agricultural University, Peshawar, Pakistan 3Department of Botany, University of Peshawar, Peshawar, Pakistan *Corresponding author e-mail: drhahmad@gmail.com |
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