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Pak. J. Bot., 44(4): 1319-1323, 2012.

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CHROMOSOME  ANALYSIS  AND  MAPPING  OF  RIBOSOMAL  GENES BY FLUORESCENCE IN SITU HYBRIDIZATION (FISH) IN FOUR ENDEMIC LILY SPECIES (LILIUM) IN QINLING MOUNTIANS, CHINA

XIANZHI WANG^1†, SONGLIN XIE^2†, YANLONG ZHANG^1* AND LIXIN NIU²

Abstract: Detailed karyotypes of 4 lily species (Lilium regale, L. duchartrei, L. brownii var viridium and L. leucanthum var. centifolium), native to Qinling Moutains in China, were constructed on the basis of chromosome arm lengths and fluorescence in situ hybridisation (FISH) with the 45S and 5S rDNA sequences as probes. Results showed that the relative length of these 4 genomes were all around 300μm, and the karyotypes were similar, which consisted of 2 pairs of (sub) metacentric and 10 pairs of (sub)telocentric chromosomes. FISH results showed that 4 pairs of homologous chromosomes with 6 45s rDNA and 2 5s rDNA signals in L. regale, 7 pairs of homologous chromosomes with 12 45s rDNA and 4 5s rDNA loci in L. duchartrei, 5 pairs of homologous chromosomes and one of the chromosome number 2 with 7 45s rDNA loci and 4 5s rDNA loci in L. brownii var viridium, and 3 pairs of homologous chromosomes with 4 45s rDNＡloci and 2 5s rDNA loci in L. leucanthum var. centifolium could be distinguished. The odd number of 45s rDNA signals in L. leucanthum var. centifolium reflected its heterozygosity.

¹College of Forestry, Northwest A&F University, Yangling Shaanxi Province 712100, P. R. China

²College of Horticulture, Northwest A&F University, Yangling Shaanxi Province 712100, P. R. China

^*Corresponding author’s e-mail: zhangyanlong@nwsuaf.edu.cn; Tel: +8613892816263
^†The authors contributed equally to this work