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Pak. J. Bot., 44(3): 1171-1174, 2012. |
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Updated: 04-06-12 | ||||
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BIOSYNTHESIS OF L-PHENYLACETYLCARBINOL FROM LOCALLY ISOLATED YEASTS
MUJAHID HUSSAIN, IKRAM-UL-HAQ, SIKANDER ALI AND M.A. QADEER
Abstract: In the present study, 250 yeast strains were isolated from
samples of different natural sources as cane-molasses, decaying
vegetables and bagasse using glucose enriched medium. Among these, 106
strains showed no growth in acetaldehyde (1 g/l) supplemented yeast
extract-peptone dextrose plates during qualitative screening. In the
course of quantitative screening, 64 acetaldehyde tolerants gave
almost negligible L-PAC production (£0.5
g/l) using glucose-peptone medium in shake flasks. A comparatively
better L-PAC production was observed with the rest of strains. The
isolate Saccharomyces cerevisiae GCU-36 exhibited higher L-PAC
production (2.58 g/l). However, lower sugar consumption and subsequent
biomass formation was noted. Therefore, yeast GCU-36 was selected as a
hyperproducer of L-PAC in batch culture.
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