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IN VITRO RESPONSE OF
RUELLIA LINEARIBRACTEOLATA TO DIFFERENT GROWTH HORMONES - AN ATTEMPT TO
CONSERVE AN ENDANGERED SPECIES
HAIDER ABBAS1*
AND MUHAMMAD QAISER2
Abstract:
Ruellia
linearibracteolata Lindau is an endangered taxon in the southern region
of Pakistan. Being exposed to harsh environmental conditions and
subjected to multiple threats including habitat loss, grazing activities
and soil erosion, Hence an urgent conservation strategy is required to
avoid its extirpation. In this connection an experiment has been
conducted to study its response to different growth hormones. Seeds from
the wild were germinated in vitro and then aseptically grown seedlings
were used as a source of explant. The efficiency of MS medium was
thoroughly examined by augmenting different growth hormones. The
cultures maintained in appropriate conditions with subculture after 6-7
months. 1.5 mg/l BAP along with 0.5 mg/l Kin and 0.5 mg/l NAA produced
the highest number (5.80) of shoots with 58.0% shoot regeneration
frequency. While, IBA (1.0 mg/l) produced maximum number (2.2) of roots
along with the highest rooting frequency (95%).
1Department
of Agriculture, University of Karachi, Karachi-75270, Pakistan,
2Federal
Urdu University of Arts, Science and Technology, Karach-75300, Pakistan
Corresponding
author’s e-mail: invitro.life@gmail.com
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