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IN VITRO
ANALYSIS OF CALLUS
INDUCTION IN INTERSPECIFICALLY HYBRIDIZED F4-5 POPULATIONS OF
BRASSICA
BILAL JAVED1, FARHATULLAH1*,
SAFDAR HUSSAIN SHAH1 AND IFTIKHAR ALI2
Abstract:
Explants of F4-5
populations of 3 B. napus (A-20-80, Maluku and Dunkled)
interspecifically hybridized with 3 B. campestris lines (1203,
2163 and 2065) were cultured on MS-medium + 2, 4- D (0.5 mg l-1)
+ Kinetin (0.25 mg l-1) for In vitro analysis. On
average, frequency of callus induction was better in F4-5
populations and B. napus species than B. campestris for
seed, cotyledonary and leaflet explants. The B. napus parental
lines were better than F4-5 population in inducing callus in
explants of leaflets and cotyledons. The callus induction was the
earliest in F4-5 population as compared to both B. napus
and B. campestris species. Callus induction in seed explants of
B. napus was late, while early in leaflet and cotyledonary
explants as compared to B. campestris.
1Department of Plant Breeding and Genetics, KP Agricultural University
Peshawar Pakistan
2Nuclear Institute for Food and Agriculture Peshawar Pakistan
Corresponding author
e-mail: drfarhat@aup.edu.pk
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