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MICROPROPAGATION OF MUSA ACUMINATA X M. BALBISIANA CV.
PISANG AWAK
(ABB GENOME) AND THREE OTHER CULTIVARS
AU VUN
HUI, ARVIND BHATT AND CHAN LAI KENG*
Abstract:
A comparative study on
three clonal propagation systems was carried out on banana cv. Pisang
Awak for production of plantlets. The temporary immersion system (TIS)
was found to be the most suitable method for in vitro propagation
of Pisang Awak plantlets
as compared to the gelled medium and the shake flask system.
There was high shoot proliferation and more roots
formation when they were cultured in MS medium supplemented with 5.0
mg/L BA in TIS. The plantlets produced from gelled medium system and
TIS also acclimatized well in greenhouse except those derived from the
shake flask system where none of the plantlet survived after transferred
to the soil. The optimized TIS propagation protocol was found to be
effective for mass propagation of other three banana cultivars, Pisang
Raja, Pisang Rastali and Pisang Berangan.
Plant Tissue and
Cell Culture Laboratory, School of Biological Sciences, Universiti Sains
Malaysia, 11800 Penang, Malaysia
Corresponding
author*: Chan Lai Keng, E-mail:
lkchan@usm.my
or
merrilynchan@gmail.com
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