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Pak. J. Bot., 44(1): 459-463, 2012. |
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Updated: 03-02-12 |
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ANTIMICROBIAL ACTIVITY OF NICOTIANA TABACUM USING DIFFERENT SOLVENTS EXTRACTS
JEHAN BAKHT1*, AZRA1 AND MOHAMMAD SHAFI2
Abstract:
The
present study investigates antibacterial activity of tobacco extracts
from Nicotiana tabacum at different concentrations in different
polar solvents. Six different extracts were prepared, using 5 different
polar solvents viz., Ethanol, Ethyl acetate, n-Hexane, Acetone, Butanol
and water. Four different concentrations (6, 12, 18 and 24 mg of sample
disc-1) of each extract were subjected for preliminary
antibacterial screening against seven pathogenic bacteria by Kirby-Bauer
Disk Diffusion method. The result of in vitro antibacterial
screening showed that 6 extracts from Nicotiana tabacum revealed
different ranges of antibacterial activities. Ethyl acetate extracted
samples were more effective to control Bacillus cereus and
Erwinia carotovora followed by butanol extracted samples against
Staphlococcus aureus and Agrobacterium tumefaciens, while no
significant inhibitory effects were observed in ethanol and hexane
extracts. When tobacco extracts were studied for their antibacterial
potential against gram-positive and gram-negative bacteria, the ethyl
acetate extracts showed a good range of inhibitory effects against
Bacillus cereus and Erwinia carotovora at highest
concentration (24 mg sample disc-1). Hexane, ethanol and
aqueous extracts did not show a significant range of inhibitory effect
but acetone extracts indicated non significant inhibitory effects
against S typhae, Staphlococcus aureus and Erwinia carotovora.
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