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Pak. J. Bot., 44(1): 445-452, 2012. |
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Updated: 03-02-12 |
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PRODUCTION OF ANTITUMOR ANTIBIOTIC BY STREPTOMYCES CAPOAMUS
HAMID MUKHTAR*, SIDRA IJAZ AND IKRAM-UL-HAQ
Abstract:
The present study is concerned with the production of antitumor
antibiotic by Streptomyces capoamus in batch fermentation.
Antibiotic activity was tested against Bacillus subtilis by
cylinder plate method. Different culture media were screened and M3
medium consisting of glucose (10.0 g/L), yeast extract (1.0 g/L), meat
extract (4.0 g/L), peptone (4.0 g/L) and NaCl (2.0 g/L) was found to be
the best. Optimum temperature, pH and incubation period for the
production of antitumor antibiotic were found to be 30oC, 7.5
and 72 hrs, respectively. 2% maltose as carbon source, 2% corn steep
liquor as nitrogen source and 48 hrs old inoculum at a concentration of
8% (v/v) were found to be the best for antitumor antibiotic production
by Streptomyces capoamus NRRL B3632. Institute of Industrial Biotechnology, GC University, Lahore 54000, Pakistan *Corresponding author: hamidwaseer@yahoo.com |
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