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Pak. J. Bot., 44(1): 365-372, 2012. |
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Updated: 03-02-12 |
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ACINETOBACTER CALCOACETICUS AMELIORATED PLANT GROWTH AND INFLUENCED GIBBERELLINS AND FUNCTIONAL BIOCHEMICALS
SANG-MO KANG1, ABDUL LATIF KHAN1,2, MUHAMMAD HAMAYUN3, ZABTA KHAN SHINWARI4, YOON-HA KIM1, GIL-JAE JOO5 AND IN-JUNG LEE1*
Abstract:
Acinetobacter
calcoaceticus,
a plant
growth promoting rhizobacteria (PGPR) and gibberellins producing strain
was investigated for the presence of organic acids in its culture and
interactive effect on cucumber plant’s growth, phytohormonal levels and
functional biochemicals. A. calcoaceticus culture’s analysis
showed the presence of three organic acids viz. malic, succinic and
citric acids. The quantity of malic acid was significantly high compared
to succinic and citric acids. Besides that, the free phosphate level was
highest at the fifth day of bacterial culture growth. In A.
calcoaceticus and plant’s association experiment, the strain has
significantly ameliorated cucumber plants to higher growth. The PGPR
culture application had higher shoot length; plant biomass and
chlorophyll contents compared to controls (distill water-DW and nutrient
broth-NB). The bacterial culture treated plants has higher amino acid
and crude protein contents compared DW and NB. In amino acid analysis,
threonine, alanine and proline quantities were significantly higher in
PGPR treated plants than control plants. A. calcoaceticus treated
plants had low endogenous abscisic acid contents but contrarily higher
GAs (GA1, GA4, GA9 and GA20)
compared to controls (DW and NB). The PGPR has activated the GAs
biosynthesis pathway while promoting the cucumber plant’s growth.
Application of such eco-friendly PGPR can be a viable alternative to
synthetic fertilizers. 1School of Applied Biosciences, Kyungpook National University, Daegu, Republic of Korea 2Department of Plant Sciences, Kohat University of Science & Technology, Kohat, Pakistan 3Department of Botany, Abdul Wali Khan University, Mardan, Pakistan 4Department of Biotechnology, Quaid-i-Azam University, Islamabad, Pakistan 5Institute of Agricultural Science and Technology, Kyungpook National University, Republic of Korea *Corresponding author: Prof. Dr. In-Jung Lee ijlee@knu.ac.kr |
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