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Pak. J. Bot., 44(1): 355-359, 2012. |
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Updated: 03-02-12 |
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BIOLOGICAL SCREENING OF OILS FROM IMPATIENS BICOLOR ROYLE
MUGHAL qayum1*, Muhammad nisar2, MUHAMMAD RAZA shah3, M. ZIA-Ul-Haq4, Waqar Ahmad KALEEM1 AND INAMULLAH KHAN MARWAT1
Abstract: The oil sub-fractions obtained by solid phase extraction of the n-hexane fraction from aerial parts of the Impatiens bicolor Royle, were tested In vitro for their antibacterial, antifungal, insecticidal, cytotoxic and phytotoxic activities. Bacterial strains used for the experiment were Shigella flexeneri, Escherchia coli, Bacillus subtilis, Pseudomonas aeruginosa, Salmonella typhi and Staphylococcus aureus while fungal strains included Candida albicans, Aspergillus flavus, Fusarium solani, Microspoum canis, Candida glaberatan and Trichophyton longifusis. The tested samples did not display any activity against above mentioned bacterial strains while in case of antifungal activity, fractions MQ-6 and MQ-7 exhibited low activity against Microsporum canis. The crude oil sub-fraction MQ-4 (81.25%) exhibited highly significant phytotoxicity in the tested concentration (1000 mg/mL) against Lemna plant. The tested samples revealed low to moderated insecticidal activity against Tribolium castaneum while in case of brine shrimp lethality, MQ-5, MQ-6, MQ-7 showed significant cytotoxicity at high dose 1000 µg/mL with LD50 value 170.748, 170.79, 161.54 µg/ mL respectively.
1Department of Pharmacy, University of Peshawar, Peshawar, 25120, Pakistan 2Institute of Chemical Sciences, University of Peshawar, Peshawar-25120, NWFP, Pakistan 3H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan 4Department of Pharmacognosy, University of Karachi, Karachi-75270, Pakistan |
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