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BIO-REACTIVE
PROPERTIES OF CITRUS WASTE: AN INVESTIGATION OF ANTIOXIDANT AND
TYROSINASE INHIBITORY ACTIVITIES
MUSARRAT AKHTAR1,
LUBNA IQBAL2*, MEHREEN LATEEF2, BUSHRA NAWAB1, MUHAMMAD SALEEM2
AND NIGHAT AFZA1
Abstract:
The antioxidant
potential as well as tyrosinase inhibitory activity of citrus fruit
waste were determined. Fruit peels of 4 species of genus citrus viz.
Citrus sinensis (Malta), C. reticulate (Orange), C.
paradisi (Grape fruit) and C. aurantifolia (Lemon), were
analyzed for these bio-reactive properties. Six different fractions of
crude extracts of each citrus fruit peel were screened for antioxidant
behavior through DPPH scavenging activity, while tyrosinase inhibition
capacity was evaluated by using kojic acid as standard tyrosinase
inhibitor. It was revealed that significant antioxidant potential was
found in chloroform fraction of malta, orange and lemon, while
chloroform and ethyl acetate, both fractions of grape fruit had 90%
antioxidant activity at 1% concentration. Alcoholic fractions of orange,
malta, and lemon showed 90, 87, and 69% tyrosinase inhibition
respectively, while no tyrosinase inhibition was found in any fraction
of grape fruit.
Food & Marine Resources Research Centre, PCSIR Laboratories Complex,
Karachi-75280
2 Pharmaceutical Research Centre, PCSIR Laboratories Complex,
Karachi-75280
*Corresponding
authors e-mail: m_saleemqazi@yahoo.com
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