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EFFECT OF GA AND ABA
ON GERMINATION BEHAVIOR OF BLACK RASPBERRY (RUBUS
COREANUS MIQUEL) SEEDS
SHAFIQ REHMAN1*,
HEH-RAN CHOI2, MUHAMMAD JAMIL3 AND SONG JOONG YUN4*
Abstract: Black raspberry (Rubus
coreanus Miquel) seed is covered with hard seed coat and enveloped in an
endospermic layer. Seeds failed to germinate without scarification and
stratification, suggesting that seeds exhibit profound both
physical and physiological dormancy.
Scarification with sulfuric acid for 15 minutes and stratification for
45 days produced best results. The application of GA was not effective
in alleviating dormancy compared to GA-untreated seeds. R.
coreanus seeds in contrast to other plant species, in which radicle
emerges first during germination process, seed coat cleaved at the
center or little more toward radicle end and whole seed emerged out
(cotyledons plus radicle) as if the chick hatched out of egg. Normally
seed germination is defined based on the radicle emergence but in case
of R. coreanus due to lack of radicle emergence seed was considered
germinated when half or more than half of the seed (radicle plus
cotyledons and some time endospermic layer) emerged from seed coat.
Furthermore, seed germination was completed in two steps, (1) cracking
of seed coat and (2) breaking of endospermic layer. The rupturing of
endospermic layer was critical in emergence of cotyledons or radicle.
The role of ABA in breaking the seed coat and endospermic layer and
effect of H2SO4 vs. manual scarification is
discussed in this article.
1Department of Botany, Kohat University of Science & Technology (KUST),
Kohat, Pakistan
2Black
Raspberry Research Institute, Gochang, Korea
3Department
of Biotechnology and Genetic Engineering, Kohat
University of Science &
Technology (KUST),
Kohat
4Department
of Crop Science, Chonbuk National University, Jeonju 561-756, Republic
of Korea
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