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DETERMINATION OF GENETIC DIVERSITY OF DIFFERENT BARLEY GENOTYPES GROWN IN
KHYBER PAKHTUN KHWA PROVINCE USING RAPD MARKERS
JEHAN BAKHT1*, MEHNAZ GHAFFAR2,
MOHAMMAD SHAFI3 SALEEM KHAN2 AND BDUL LATIF4
Abstract:
Plant breeders in Pakistan mostly relied upon phenotypic traits as
indices of genetic variation among different varieties which may
genotypically be closely related or vice versa. The main objective of
the current study was to determine genetic diversity among 12 different
barley genotypes grown in Khyber Pakhtun Khwa province of Pakistan, The
results indicated that different barely genotypes showed various levels
of polymorphism for the loci detected by seven RAPD primers. A total of
205 alleles were observed giving an average of 17.08 bands genotype-1.
Among the seven primers used in the current study, primers C06 yielded
on an average maximum number of bands (3.3) genotype-1. While
primer G05 produced on an average minimum number of bands (1.75)
genotype-1. Range of genetic distance observed was 0 to 60%.
Maximum genetic distance was observed for genotype serial number 4 and
11 while 11 and 12 showed no genetic distance. Total average genetic
distance calculated for all seven RAPD primers was 41.26%. Three major
groups with at least two sub-groups were identified among these barely
genotypes. Genotype serial number 3, 4 and 5 were found to be
genetically related with a genetic distance of 13.0% and genotype serial
number 4 and 11 were genetically very distant with 60% genetic distance.
On the basis of dendrogram, these genotypes were grouped into three
groups. (1) Group A comprises of genotype serial number 7 and 8 (2)
Group B comprises of genotype serial number 2, 3, 4, 5, 9, 10, 10, 11
and 12 (3) Group C genotype serial number 1 and 6.
1Institute
of Biotechnology & Genetic Engineering, KPK Agricultural University
Peshawar, Pakistan
2Agricultural
Research Station Baffa Mansehra, KPK Pakistan
3Department
of Agronomy, KPK Agricultural University Peshawar, Pakistan
4Barani
Agricultural Research Station Kohat, KPK Pakistan
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