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STUDIES ON THE EFFECT
OF GENOTYPE AND EXPLANT TYPE ON CALLOGENESIS AND
ORGANOGENESIS IN INDICA RICE
AMNA NOOR1, HAMID RASHID2, MOHAMMAD
HAROON KHAN2 AND ZUBEDA CHAUDHRY3
Abstract:
The purpose of the research study is to select the best rice explant and
variety for Agrobacterium mediated genetic transformation
studies. In vitro callus induction and regeneration
frequency of two explants i.e. immature embryo and mature grains of 9
rice varieties (Oryza sativa L.) was investigated by
culturing explants source on N6 media supplemented with 2,
4-D at 2mg/l for callus induction. Results indicated that mature seed
explant produced significantly high number of calli as compared to
immature embryo. Two types of calli were distinguished, designated as
type-I calli and type-II calli. Regarding the quality of callus, type-I
calli (produced from scutellum of mature grains) which were embryogenic
produced higher plant regeneration frequency than type-II calli which
were non-embryogenic. Mature-seed scutella calli and immature
embryo-derived calli (after three weeks of culture) of rice varieties
were transferred on regeneration medium i.e., MS salts and vitamins, 3%
sucrose, 3% sorbitol, 2g/l casine hydrolysate, NAA 1.0 mg/l, kin 2.5 mg
/l and BAP at 0.5mg/l. The highest regeneration capacity was observed in
DR-83 from mature seed derived calli followed by Basmati 385. After
hardening the plantlets were transferred to soil.
1Agriculatural
Biotechnology, Program NARC Islamabad, Pakistan
2Department
of Bioinformatics, Mohammad Ali Jinnah University, Islamabad, Pakistan
3Department
of Botany, Hazara University, Mansehra, NWFP, Pakistan.
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