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PLANT REGENERATION FROM
IRRADIATED EMBRYOGENIC CALLUS OF SUGARCANE
SHAFQUAT YASMIN,
IMTIAZ A. KHAN, ABDULLAH KHATRI, NIGHAT SEEMA, M.
AQUIL SIDDIQUI AND SAJIDA BIBI
Abstract:
Three sugarcane
clones viz., NIA-98, NIA-0819 and BL4 were used for induction of genetic
variability through In-vitro mutagenesis. Apical meristametic
region was used for callus induction on 4mg/l 2,4-D. Actively growing
callus was treated with four different doses of gamma rays (10Gy, 20Gy,
30Gy and 40Gy). Maximum callus, proliferation and plantlets regeneration
growth was observed in control and minimum at 40Gy. Maximum chlorophyll
mutation frequency was recorded in 30 and 40 Gy. Maximum number of roots
was observed in control followed by 10Gy. The maximum root length
(10.3cm) was obtained in control at 2 mg/l IBA. The maximum number of
tiller in the irradiated population was observed in 10Gy. The treatments
30Gy and 40Gy exhibited negative impact on the tillering potential of
the plant.
Nuclear Institute
of Agriculture, Tando Jam, Pakistan
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