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Assessment of
relocation of Trigonella cylindraceae l. and T.
polyceratia (l.) trautv. to genus Medicago as inferred by RAPD and
RFLP analyses
REAM I. MARZOUK1
AND EL-BAKATOUSHI R.2*
Abstract:
The
taxonomic relocation of both Trigonella cylindraceae and T.
polyceratia either to Trigonella or Medicago was
assessed based on two molecular markers, RAPD and RFLP. Through RAPD
analysis, 109 bands were generated by ten random primers with an average
11 products/primer. The maximum number of RAPDs (76 bands) associated
with the highest percentage of polymorphism (43.3%) was recorded for
T. polyceratia, while the minimum was observed in T.
foenum-graecum (63 bands and 32.3%). RFLP analysis of 18S rDNA was
performed by using five restriction endonucleases (EcoRI, HindIII,
BamHI, HinfI and TagI). The restriction endonuclease HinfI and TagI
produced two and three fragments respectively, and no variation was
observed in these sites among the studied species. However, another
three restriction endonuclease (EcoRI, HindIII & BamHI) were undigested.
The present study suggested to reallocate of both T. cylindraceae
and T. polyceratia to Medicago and certified the value of
RAPD analysis for the taxonomic intention.
1Department
of Botany, Faculty of Science, Alexandria University, Egypt
2Department
of Biological and Geological Sciences, Faculty of Education, Alexandria
University, Egypt.
*E-mail: ranyaelbakatoushi@yahoo.com
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