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GENETIC DIVERSITY OF NURYT-NATIONAL UNIFORM RAPE SEED YIELD TRIAL AND BRASSICA NAPUS VARIETIES USING RAPD MARKERS AND BIOCHEMICAL ANALYSIS
JEHAN BAKHT1, AMNA FAREED1,
MOHAMMAD SHAFI2, ZAHOOR AHMAD SWATI1
Abstract: In
Pakistan, Brassica is the second most important source of oil
after cotton. Seventeen NURYT (National Uniform Rape Seed Yield Trial)
lines and 5 Brassica napus varieties were assessed through RAPD
primers and biochemical assays. Seven different Randomly Amplified
Polymorphic DNA markers (RAPD) were employed during the present study. A
total of 30 RAPD bands were scored by these primers. Size of the
scorable fragments ranged from approximately 250 to 2000 bp. Diversity
index was estimated to be 42%. Mean genetic distance estimates ranged
between 0.10 and 1.00. For the assessment of various biochemical
parameters, Near Infrared Reflectance Spectroscopy (NIRS) was used. Oil
content ranged from 38.30 to 49% and protein content from 19.80 to
29.10% among the 22 genotypes. Maximum protein content was assayed in
genotype RBN 3046 while minimum in Hyola 405. Glucosinolates ranged
between 2 and 84% for genotype CRH 60/08 and CRH05/08 showing the
maximum and minimum values respectively. Oleic acid (52 to 72.5%), linolenic acid (7.07 and 9.90%) and erucic acid content (9.57 to 38.3%)
was also recorded during the present study.
1Institute
of Biotechnology & Genetic Engineering, KPK Agricultural University
Peshawar, Pakistan
2Department
of Agronomy, KPK Agricultural University Peshawar, Pakistan
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