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INFLUENCE OF PHYTOHORMONE ON THE ORGANOGENESIS OF SUGARCANE

NIGHAT SEEMA, FATEH CHAND OAD^*, IMTIAZ AHMED KHAN, *SHAMSSUDDIN TUNIO, MOHAMMAD AQUIL SIDDIQUI SHAFQUAT YASMIN, ABDULLAH KHATRI AND SAJIDA BIBI

Abstract: Three sugarcane (Saccharum sp. Hybrid) varieties viz., AEC82-1026, NIA-98 and BL4 were used in this study in order to compare their response to different concentration of phytohormones (Dicamba 2,4D and Picloram). Best callus induction was observed using dicamba fallowed by 2,4-D whereas best proliferation was achieved with dicamba. The maximum callus formation, and plantlets regeneration were recorded in AEC82-1026 and minimum in BL4.The growth regulators dicamba and 2,4D induced more genetic variability as compared to picloram. After regeneration, the plantlets were transferred on eight different rooting medium and best rooting was observed on media containing 2mg/l IBA with 4% sugar. The AEC82-1026 produced maximum number of roots secondary roots followed by NIA 98. The well rooted plantlets were transferred to the green house and than into the field for evaluation.   

Nuclear Institute of Agriculture, Tando jam, Sindh Agriculture University, Tando Jam

^*Sindh Agriculture University, Tando Jam