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Effects of Stratification at Different Temperatures on the Embryo Differentiation and Development of Paris polyphylla var. yunnanensis seeds

Yu Ye, Shu-ying Chen, Li Wang*, Yan-qiong Yang and Chang-ling zhao

Abstract: Effects of stratifications at 6 temperatures on the embryo differentiation and development of Paris polyphylla var. yunnanensis seeds were studied by using photomicrography based on paraffin section. The results indicated that there were striking differences among the differentiating and developing processes of the embryos of P. polyphylla var. yunnanensis seeds stratified under different temperatures. The stratifications alternating between 15^oC and 20^oC promoted the embryo differentiation and development best, next are the stratifications at 15^oC and 20^oC, the stratification at 10^oC did not display obvious effects and the stratifications at 5^oC and 25^oC showed inhibiting effects to some extent. Additionally, the embryos differentiated and developed slowly under natural conditions. This research could provide a basis for the dormancy-breaking of Paris polyphylla var. yunnanensis seeds.

College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming 650201, China

* Corresponding author E-mail: wangli5820840@yahoo.com.cn