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EFFECT OF DIFFERENT MEDIA AND SOLIDIFYING AGENTS ON CALLOGENESIS AND PLANT REGENERATION FROM DIFFERENT EXPLANTS OF RICE (ORYZA SATIVA L) VARIETIES SUPER BASMATI AND IRRI-6

HUMERA AFRASIAB* AND RABIA JAFAR

Abstract: In the present work, plant tissue culture conditions were optimized for In vitro germination from dehusked seeds of two varieties of rice (Oryza sativa L), Super Basmati and IRRI-6 on MS and LS medium solidified with agar and phytagel. MS medium solidified with agar was optimum for In vitro germination of Super Basmati and LS medium containing agar was best for germination of IRRI-6. For best callus induction and proliferation from mature embryos of both varieties and leaf bases of IRRI-6, MS medium supplemented with 2.0 mg/l 2, 4-D was efficient, while leaf bases of Super Basmati required a higher concentration of 2,4-D. For efficient regeneration from callus, MS medium supplemented with NAA 1.0 mg/l with BAP 3.0 mg/l proved better for Super Basmati and for IRRI-6, MS medium containing NAA 1.0 mg/l and BAP 5.0 mg/l showed better results. Light conditions proved better for callogenesis and regeneration from mature seed explants of both the varieties in MS medium supplemented with 2.0 mg/l 2, 4- D.

Department of Botany, University of the Punjab, Lahore, Pakistan.

*Corresponding author E-mail: nida13pk@yahoo.com