| Pak. J. Bot., 43(1): 467-473, 2011. | Back to Contents | ||||
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Updated: 26-02-11 | ||||
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CALLUS INDUCTION AND CELL SUSPENSION CULTURE PRODUCTION OF CATHARANTHUS ROSEUS FOR BIOTRANSFORMATION STUDIES OF (–)- CARYOPHYLLENE OXIDE
SAIFULLAH* AND SAIFULLAH KHAN
Abstract:
For biotransformational studies, Callus of Catharanthus roseus
was produced from young shoot tip on Murashige & Skoog medium
supplemented with 1.5 mg/litre 2, 4-D and 0.5 mg/litre Kin, solidified
by 8 g/litre agar at 25±1°C under complete darkness. The cultures were
maintained on the same medium at 25±1 °C in the dark by subculturing
after every four weeks. Suspension culture of the plant was produced
from induced callus on the same medium except Kin and agar. Cell
suspension culture of Catharanthus roseus (Madagascar periwinkle)
was employed for the first time in order to study the
biotransformational capability of (–)-caryophyllene oxide (1),
and four metabolites, 15-hydroxycaryophyllene oxide (2), 4β,
5α-dihydroxycaryophyll-8(13)-ene (3), 2β-hydroxycaryophyllene
oxide (4), and 2-hydroxy-4,5-epoxycaryophyllan-13-ol (5)
were obtained. Metabolites 4 and 5 were found to be new
compounds. H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan |
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