Pak. J. Bot., 42(6): 4253-4266, 2010.

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PROFILING GENE EXPRESSION PATTERNS OF STRIPE RUST (PUCCINIA STRIIFORMIS F.SP.TRITICI) RESISTANCE GENE IN NEW WHEAT GERMPLASM

ZENG XINGQUAN^{1, 2, †}, WANG CHANGYOU^{1, †}, M. ALI^1,3, ZHANG HONG¹, LIU XINLUN¹, LI WEIYAN¹ AND JI WANQUAN^1,*

Abstract: Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst), is one of the most devastating diseases in common wheat (Triticum aestivum L.) worldwide. Total RNA for SSH analyses was extracted from wheat germplasm NR1121 infected by Chinese Pst race CYR32. To identify differentially expressed genes in response to Pst infection in wheat, all 550 Positive clones from the library were subjected to sequencing, while 162 expressed sequence tags (ESTs) were to clustering, BLAST alignment, functional annotation and classification into different categories. The expression of eight genes was detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time polymerase chain reaction (real time-PCR). Expression of  six genes (acyl-coenzyme A synthetase, glutathione S transferase, Lipid transfer protein, ubiquitin protein ligase 2, cytochrome P450, and serine/threonine protein kinase SNT7) transcripts were induced and up-regulated to their highest levels at 24 hours after Pst infection, while that of two genes(serine Hydroxy-methyl transferase and S-adenosylmethionine decarboxylase) were significantly expressed at 48 hours (hpi). These genes were highly induced at an early stage of infection, suggesting they are transcriptionally activated for the host defense response.

¹College of Agronomy, Northwest A&F University, Yangling, Shaanxi 712100, China

²Tibet Academy of Agriculture and Animal Husbandry Sciences, Lhasa, Tibet 850002, China

³Cotton Section Agriculture Research Institute, Tandojam, Pakistan