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OPTIMIZATION OF PROCESS PARAMETERS FOR THE BIOSYNTHESIS OF CELLULASES BY TRICHODERMA VIRIDE

SHAZIA KANWAL MALIK¹, HAMID MUKHTAR¹, AMMAD AHMAD FAROOQI² AND IKRAM-UL-HAQ¹

Abstract: Present studies describe the optimization of process parameters for the production of cellulases by Trichoderma viride using submerged fermentation technique. The fermentation experiments were carried out in shake flasks using pretreated bagasse. Maximum production of cellulases (CMCase 1.57 U/ml/min, FPase 0.921 U/ml/min) was observed after a fermentation period of 72 hrs at an incubation temperature of 30ºC. Initial pH of the culture medium was also optimized and a pH of 5.5 was found to support maximum growth and enzyme production (CMCase 1.66 U/ml/min and FPase 0.932 U/ml/min) by T. viride. Different inorganic nitrogen sources were evaluated for the production of cellulases and Ammonium sulphate was found to be the best. The enzyme production was further enhanced by carrying out fermentation experiments using 25 ml of culture medium in 250 ml flask inoculated with 4% conidial inoculum.

¹Institute of Industrial Biotechnology, GC University, Lahore 54000, Pakistan

²Institute of Molecular Biology and Biotechnology, The University of Lahore, Pakistan.