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Pak. J. Bot., 42(6): 4063-4069, 2010.

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BIOLOGICAL SCREENING OF ZIZYPHUS OXYPHYLLA EDGEW LEAVES

MUHAMMAD NISAR*¹, WAQAR AHMAD KALEEM², MUGHAL QAYUM², ABRAR HUSSAIN⁴, M. ZIA-UL-HAQ⁵, IHSAN ALI¹ AND M. I. CHOUDHARY³

Abstract: Crude methanolic extract and various fractions of Zizyphus oxyphylla Edgew leaves were screened for antibacterial, antifungal, phytotoxic, cytotoxic and insecticidal activities. Antibacterial bioassay was performed against six bacteria viz., Escherichia coli, Bacillus subtilis, Shigella flexenari, Staphylococcus aureus, Pseudomonas aeruginosa, and Salmonella typhi . Ethyl acetate fraction showed good antibacterial activity against Bacillus subtilus (16mm zone of inhibition) and Staphylococcus aureus (18 mm zone of inhibition). The antifungal activity of these extract performed against 6 fungal strains viz., Trichophyton longifusus, Candida albicans, Aspergilus flavus, Microsporum canis, Fusarium solani and Candida glaberata indicated non significant results. Similarly neither of the fractions showed any cytotoxicity while n-hexane and ethyl acetate fraction showed moderate (40 %) insecticidal activity. All fractions and crude extract showed significant (60-90 %) phytotoxicity.

¹Institute of Chemical Sciences, University of Peshawar, Peshawar-25120, NWFP, Pakistan

²Department of Pharmacy, University of Peshawar, Peshawar, 25120, Pakistan

³H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences, University of Karachi, Karachi-75270, Pakistan

⁴Quality Control Department, Wilson’s Pharmaceuticals, Islamabad, Pakistan

⁵Department of Pharmacognosy, Wilson’s Pharmaceutical, Islamabad, Pakistan