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Pak. J. Bot., 42(6): 3839-3845, 2010. |
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Updated: 23-12-10 | ||||
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CHARACTERIZATION OF BREEDING LINES OF COMMON BEAN AS REVEALED BY RAPD AND RELATIONSHIP WITH MORPHOLOGICAL TRAITS
ATILLA DURSUN1*, KAMIL HALILOGLU2 AND MELEK EKINCI1
Abstract: Different type of data can be used to estimate genetic diversity. In this work, genetic diversity among common bean breeding lines by random amplified polymorphic DNA (RAPD) markers, and morphological traits to analyze differences among common bean breeding lines were studied and usability of RAPD markers for estimation of genetic diversity among common bean breeding lines in comparison with morphological traits were evaluated. Eight RAPD markers generated polymorphic patterns, yielding a polymorphism rate of 80%. The average genetic similarity among the common bean breeding lines was 0.55 with values ranging from 0.19 between H-166 and H-212 and 0.67 between H-212 and H-128 breeding lines, having the highest genetic similarity. In the principal component analysis (PCA), the first three principal components explained about 81% of the variation in morphological traits. A Mantel’s test showed low correlation between RAPD and morphological data distance matrices.
1Department of Horticulture, Faculty of Agriculture, Ataturk University, Turkey, 25240 Erzurum 2Department of Agricultural Biotechnology, Faculty of Agriculture, Ataturk University, Turkey, 25240 Erzurum *Corresponding author: E-mail address: atilladursun@hotmail.com |
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