|
Pak. J. Bot., 42(6): 3783-3790, 2010. |
Back to Contents | ||||
|
|
Updated: 23-12-10 | ||||
|
|
GENOTYPE INDEPENDENT IN VITRO REGENERATION SYSTEM IN ELITE VARIETIES OF SUGARCANE
SAFDAR ALI1, JAVED IQBAL3 AND MUHAMMAD SARWAR KHAN2*
Abstract: Nine sugarcane genotypes were tested for callus induction and subsequent regeneration on MS medium supplemented with different combinations of hormones. Genotype-independent plant regeneration was developed from calli induced from young leaf roll discs on MS medium supplemented with seven different levels (1-5mgl-l) of 2, 4-D. Further, five levels (1-5mgl-l) of 2, 4-D alongwith 1 mgl-1 of Kinetin for callus induction were used. All the genotypes responded well to 2, 4-D alone as well as in combination with Kinetin. Nevertheless, high percentage of regeneration was observed on MS medium supplemented with 500mg l-l casein hydrolysate. Regenerated shoots developed profused roots on NAA (4-5mgl-l) and casein hydrolysate (500mgl-l)-containing MS medium which were otherwise delayed on a medium containing full as well as half strength MS salts.
1Department of Botany, Govt. College University, Lahore, 2Centre of Agricultural Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad, Pakistan, 3School of Biological Sciences, University of Punjab, Quad-e-Azam Campus, Lahore, Pakistan. Correspondence: safdaralimirza@gmail.com |
||||
|
|
|||||
| Back to Contents |
|
Back to Contents | |||
|
|
|||||