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Genetic diversity in some Tunisian barley landraces based on RAPD markers

Raoudha ABDELLAOUI^1*, Karim KADRI², M’barek BEN NACEUR³ and Leïla BETTAIB BEN KAAB⁴

Abstract: The genetic diversity analysis of 15 barley landraces was carried out using RAPD markers. These landraces were collected from various bioclimatic Tunisian zones. The amplification products varied from 4 to 11 bands ranging between 250 pb and 3000 pb. On 698 fragments counted, 578 are polymorphic showing a high level of polymorphism (82.8%). The relationship between the studied landraces was evaluated according to (UPGMA) method that classified barley landraces in 4 homogeneous groups. Among which, the group D included the majority of the landraces with the introduced variety ‘Martin’. The genetic distance between these landraces is reduced, may be because of the presence of a common ancestor which led to a narrow genetic diversity.

¹Range Ecology Laboratory, Arid Regions Institute of Medenine, Street Djerba 22.5 Km 4119 Medenine, Tunisia.

²Biotechnology and Tissues Culture Laboratory, Research regional centre of  Oase Agriculture 62 Street Tozeur Km1, Degash 2260, Tunisia.

³National Agronomic Research Institute of Tunisia, street hédi Karray 2049 Ariana, Tunisia.

⁴Faculty of Sciences of Tunis, El Manar University, Tunisia.

*Corresponding author: E-mail : raoudhamabdellaoui@yahoo.com; raoudha.abdellaoui@ira.agrinet.tn