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Pak. J. Bot., 42(6): 3765-3773, 2010. |
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Updated: 23-12-10 | ||||
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SCREENING OF MUTATED POPULATION OF SUGARCANE THROUGH RAPD
SAJIDA BIBI, IMTIAZ AHMED KHAN, ABDULLAH KHATRI, SHAFQAT YASMIN, NIGHAT SEEMA, SHAHID AFGHAN* AND M.A. ARAIN
Abstract: Variability obtained from mutation breeding (gamma rays) was examined through molecular marker techniques (RAPD). A total of 73 loci were amplified with 13 primers, out of which 75.34% were polymorphic and 24.66% were monomorphic. Fragments size ranged from 275bp-2.2kb and fragments produced by various primers ranged from 3-9 with an average of 3 fragments per primer. The highest number of loci 9 was amplified with primer B-09, while the lowest number 3 with primer B-02 and B-08. Results revealed that mutant P2(30Gy) contained three specific segment of 421bp, 2.0kb and 2.1kb while Mutant P1 (30Gy) contains another specific segment of 2.0kb amplified with primer B-06. Genetically most similar genotypes were P3 (10Gy) and P4 (10Gy) (96.5%) while most dissimilar genotypes were P2 (20Gy) and P1 (30Gy) (48%). On the basis of results achieved, the mutants could be divided into four cluster and three groups. Mutants P2 (30 Gy), P1 (20 Gy) and P2 (20 Gy) were genetically distinct from other mutants.
Plant Breeding and Genetics Division Nuclear Institute of Agriculture, Tando Jam, Pakistan *SSRI, Jhang |
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