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IN VITRO REGENERATION OF GINGER USING LEAF, SHOOT TIP AND ROOT EXPLANTS

AZRA SULTANA¹, LUTFUL HASSAN^2*, SYED DILNAWAZ AHMAD³, A.H. SHAH³, FARHAT BATOOL⁴, M.A. ISLAM¹, R. RAHMAN¹ AND S. MOONMOON¹

Abstract: MS medium supplemented with different combinations and concentrations of hormone were studied to obtain a suitable protocol of plantlet regeneration of ginger from calli. Three explants of two varieties of ginger viz., Suruchi and BARI ada-1 were cultured on MS medium supplemented with 0.5 mg/l Dicamba, 0.75 mg/l Dicamba and 1 mg/l 2,4 D. Assessments on callus induction were studied through five quantitative traits such as days required for callus initiation, size of callus, color of callus, nature of callus and percentage of callus induction. Suruchi showed 62.64% callus induction, 63.98% shoot induction and 68.76% root induction. Leaf explant gave best result over shoot tip and root explant. Leaf explant produced 67.07% callus, 67.77% shoot and 66.93% root. MS medium supplemented with 0.5 mg/l Dicamba was the best (70.20%) for callusing, MS + 1.0 mg/l Kn + 1.0 mg/l BAP best (72.03%) for shooting and Ms + 1 mg/l IBA gave best (66.43%) result for rooting over other treatments. The highest (73.60%) callus induction was found from the leaf x Suruchi interaction. Leaf x Suruchi gave 74% shooting and 74.13% rooting. Percent callus induction was maximum (76.47%) with Suruchi x 0.5 mg/l Dicamba interaction and it was significantly different from all other values. Percent shoot induction were highest (76.33%) by the interaction of Suruchi x 1.0 mg/l Kn +1.0 mg/l BAP. Highest (76.87%) percentage of root was produced by 1 mg/l IBA x Suruchi interaction. Highest percentage of callus induction (87.60%) was obtained from leaf explants of Suruchi and 0.5 Dicamba interactions. The interaction of Suruchi x leaf x 1.0 mg/l Kn + 1.0 mg/l BAP produced highest (87.60%) percentage of shoot. Root induction was best (85.40%) from Suruchi x leaf x MS + 1 mg/l IBA interaction. The regenerated plantlets were successfully established into pot after proper hardening.

¹Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh,

²Department of Genetics & Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh,

³Department of Plant Breeding and Molecular Genetics, Faculty of Agriculture, Azad, Jammu and Kashmir University, Pakistan,

⁴Neurochemistry and Biochemical Neuropharmacology Research Laboratory, Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan.