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IN VITRO REGENERATION POTENTIALITY OF OIL SEED BRASSICA GENOTYPES WITH DIFFERENTIAL BAP CONCENTRATION

MOHAMMAD MAHBUBUL ALAM KHAN¹, LUTFUL HASSAN¹, SYED DILNAWAZ AHMAD², ASAD HUSSAIN SHAH² AND FARHAT BATOOL^3*

Abstract: Petiole of six genotypes of oilseed Brassica viz., Tori-7, Sampad, Kallyania, BARI Sarisha-7, BARI Sarisha-8 and MM 20-3 were cultured in MS medium with different concentrations of BAP, NAA and AgNO₃ for callus induction and subsequent plant regeneration. The highest percentage of callus induction (91.43%) was observed in Tori-7 with the media supplemented with 2 mgL^-1BAP, 0.1 mgL^-1 NAA and 2.0 mgL^-1 AgNO₃. Calli were maintained in order to get sufficient number of regenerants. With the increased concentration of BAP, the highest percentage (57.14) of regenerants were found in Tori-7 followed by Sampad (33.13%) and BARI Sarisha-8 (31.42%) in MS medium supplemented with 2.5 mgL^-1 BAP, 0.1mgL^-1 NAA and 2.0mgL^-1 AgNO₃. Root formation from the regenerants was found best in half MS medium supplemented with 0.5mgL^-1 NAA in genotype Tori-7. Regenerated plantlets of 4 genotypes (Tori-7, BARI Sarisha-8, Kallyania, BARI Sarisha-7) were successfully established in the field.

¹Department of Biotechnology, Bangladesh Agricultural University,

²Department of Genetics and Plant Breeding, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

³Neurochemistry and Biochemical Neuropharmacology Research Laboratory, Department of Biochemistry, University of Karachi, Karachi-75270, Pakistan.
Corresponding author E-mail: batool@uok.edu.pk; Mob:  0333-3097217