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INDUCED QUANTITATIVE VARIABILITY BY GAMMA RAYS AND ETHYLMETHANE SULPHONATE ALONE AND IN COMBINATION IN RAPESEED (BRASSICA NAPUS L.).

MUHAMMAD AQUIL SIDDIQUI, IMTIAZ AHMED KHAN AND ABDULLAH KHATRI

Abstract: Genetic variability induced by gamma rays (750 Gy, 1000 Gy) and ethylmethane sulphonate (EMS) (0.75%, 1.00% solution) alone and in combination (750 Gy+0.75% EMS, 750 Gy+1.00% EMS, 1000 Gy+ 0.75% EMS and 1000 Gy+1.00% EMS) was evaluated for important economic character in rapeseed (Brassica napus L.) cv. Waster. Comparison with control revealed that the mutagen treatments shifted the mean values in positive as well as negative directions. However, all the mutagens showed enhancing effect for siliquas per plant and deteriorating effect for grains per siliqua and oil (%). Combinations of physical and chemical mutagen have shown enhancing effect on primary branches. There was a considerable increase in variance for all the traits under study. The induced variation can be exploited in the evolution of new varieties of rapeseed with improved agronomic traits.

Agriculture Biotechnology Division, Nuclear Institute of Agriculture, Tando Jam-70060, Pakistan.