| Pak. J. Bot., 40(3): 1225-1230, 2008. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
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ISOLATION OF A XYLAN DEGRADING GENE FROM GENOMIC DNA LIBRARY OF A THERMOPHILIC FUNGUS CHAETOMIUM THERMOPHILE ATCC 28076 FOUZIA SALEEM1, SIBTAIN AHMED1,2 AND AMER JAMIL1* Abstract: The most abundant hemicellulosic polymers xylans, constitutes about 20-40% of total plant biomass. Xylanases are involved in fruit softening, seed germination and plant defense systems. In this study, genomic DNA of Chaetomium thermophile ATCC 28076 was isolated and a genomic library was created in E.coli DH10B-pUC 19 host vector system. Positive clones were screened by Congo red staining. The E.coli harboring xylanase gene showed clear zone with Congo red clearance assay on xylan plates. 1Molecular Biochemistry Lab., Department of Chemistry and Biochemistry, University of Faisalabad-38040, Pakistan. 2Department of Physiology and Biochemistry, University of Veterinary and Animal Sciences, Lahore, 54000, Pakistan. *Corresponding author: amerjamil@yahoo.com |
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