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CALLUS FORMATION AND PLANTLETS REGENERATION FROM HYPOCOTYL OF BRASSICA NAPUS BY USING DIFFERENT MEDIA COMBINATIONS

MUHAMMAD MUNIR, HAMID RASHID*, M. RAUF, ZUBEDA CHAUDHRY^*  AND M. SHAHJAHAN BUKHARI

Abstract: The genus Brassica includes several important crop species. Canola (Brassica napus L.) is considered as the most important source of vegetable oil and protein rich meal worldwide. Effect of 5 different callusing media (CIM₁ to CIM₅) and regeneration media (RM₁ to RM₁₀) supplemented with different concentrations of hormones were studied for callus induction and regeneration of four cultivars of Brassica napusc viz., Dunkeld, Oscar, H-19 and Rainbow. The experimental work was carried out at the Agricultural Biotechnology Program, National Agricultural Research Centre, Islamabad during March 2004 to January 2005. The results indicated that among the 5 media combinations, MS media supplemented with 0.5mg /l IAA, 1mg /l BAP, 0.5mg/l NAA and 1 mg /l Kinetin (CIM₂) was the most responsive media and gave best results for callus induction followed by CIM₃, CIM₁, CIM₄ and CIM₅. As the concentration of IAA was increased further there was a decrease in callus induction. The cultivar Oscar formed relatively more calli at higher rates closely followed by cultivar H-19. The cultivar Rainbow showed minimum calli formation among the 4 cultivars. In case of regeneration, all the 10 media combinations showed significantly different regeneration response except RM₁ and RM₆ media combinations that showed almost no regeneration. The best results were obtained when explants were cultured on a regeneration medium containing 1mg Zeatin/l and 0.1 mg IAA/l. Maximum regeneration percentage was recorded with RM₈ followed by RM_3. The results indicated that cultivar Oscar showed regeneration relatively at higher rates. The cultivar Rainbow showed minimum regeneration capability among the four cultivars.

Department of Plant Breeding and Genetics, University of Arid Agriculture Rawalpindi, Pakistan

^*Agricultural Biotechnology Program, NARC, Islamabad, Pakistan