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IN VITRO RE-GENERATION OF GUAVA (PSIDIUM GUAJAVA L.) FROM SHOOT TIPS OF MATURE TREES

ROSHAN ZAMIR, N. ALI^*, S.T. SHAH, T. MUHAMMAD AND S.A. SHAH

Abstract: Micropropagation of guava through shoot tip culture from five years old bearing plants was carried out at Nuclear Institute for Food & Agriculture, Peshawar. Shoot tips after sterilization was cultured on modified MS medium supplemented with different concentrations and combinations of BAP and L-glutamin. Maximum number of shoots (72 %) was developed in to plantlets when MS was supplemented with BAP 1 mg/L and 500 mg/L L-glutamine. MS control and BAP 1mg/L combined with glutamine 250 mg/L gave minimum (22%) plantlets. Maximum (3.5) number of shoots per culture was found in 0.5 mg/L BAP alongwith 500 mg/L L-glutamine. MS supplemented with 2.5 mg/L IAA + 2.5 mg/L IBA gave maximum (54) plants rooted with average number of roots (3.8) per plantlet. The In-vitro produced plantlets were successfully established in soil.

Nuclear Institute for Food and Agriculture (NIFA), Tarnab, Peshawar, Pakistan

^*NWFP, Agricultural University, Peshawar, Pakistan

Correspondence e-mail: rzamir2005@yahoo.com