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MULTIPLE  SHOOT  FORMATION  FROM DIFFERENT EXPLANTS OF CHICK PEA (CICER ARIETINUM L.)

SHAGUFTA NAZ, AAMIR ALI, FAYYAZ AHMAD SIDDIQUE AND JAVED IQBAL

Abstract: The direct regeneration ability in different explants i.e., shoot and nodal explants of Cicer arietinum was investigated on M.S. basal medium supplemented with different concentrations of Benzyl amino purine (BAP). Rapid and early shoot initiation was observed in lower concentration of BAP. Higher concentrations delayed shoot initiation in both explants. The number of shoots per explant increased with BAP concentration while shoot length decreased with increasing BAP concentration. The overall response of nodal explant was better than shoot apex. The regeneration shoots were further multiplied by sub culturing on fresh medium. The multiple shoots were shifted to full and half strength M.S. medium supplemented with different concentrations of NAA and IBA for induction of roots while the half strength media with 1.0 uM NAA gave excellent response of root induction.
 

Department of Botany, Lahore College for Women University, Lahore, Pakistan.