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IDENTIFICATION AND ISOLATION OF LOW PHYTIC ACID WHEAT (TRITICUM AESTIVUM L.) INBRED LINES / MUTANTS

ABDUL JABBAR KHAN^*, AKHTAR ALI, FAROOQ-I-AZAM AND AURANG ZEB

Abstract: Research studies were carried out for identification and isolation of low phytate bread wheat mutants / cultivars in Pakistan. Sixty six mutants / cultivars collected from different parts of the country were assayed for phytic acid contents. The phytic acid contents ranged from 0.98% (Parvaz-94) to 2.17 % (NRL-0443). The frequency distribution curve showed that most of the genotypes had seed phytic acid contents in the range of 1.28 - 1.72%. Pooled analysis of revealed that seed phytic acid contents have significant correlations with seed width (p<0.001), seed thickness (p<0.01) and seed volume (p<0.05). Seven genotypes viz., Bakhtawar-92, Parwaz-94, NRL-0406, NRL-0416, NRL0431, NRL-0439 and NRL-0441 were identified as having low phytic acid contents. The selected lines were grown at 5 locations differed for soil types and environmental condition to determine G x E interaction on phytic acid contents. Cultivar Parvaz-94 gave consistently low and mutant NRL-0431 gave the highest concentration of phytic acid across the location. The effects of genotypes, environments (locations) and their interactions on phytic acid content were all highly significant, with the location having the largest effect. The highly significant interaction between genotype and environment suggests that the correct evaluation of wheat germplasm by phytic acid content should be conducted in multi-environments. 

Nuclear Institute for Food and Agriculture (NIFA) Tarnab, P.O. Box # 446, Peshawar, Pakistan.