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MYCOFLORA ASSOCIATED WITH LENTIL (LENS ESCULENTA MOENCH) SEEDS FROM FIVE LOCALITIES OF PUNJAB, PAKISTAN

MUHAMMAD ARSHAD HUSSAIN¹, TARIQ MUKHTAR^2*, M. IRFAN UL-HAQUE² AND MUHAMMAD ZAMEER KAYANI³

Abstract: Twenty five samples of lentil (Lens esculenta Moench) seed collected from the markets of Faisalabad, Dera Ghazi Khan, Bahawalpur, Jampur and Rojhan were analyzed for externally and internally seed-borne fungi. The infestation of untreated seed samples varied from 43.3 to 51.4% with an average of 46.96%, while the seeds treated with 2% Mercuric chloride solution showed the infestation from 18.0 to 22.4% with an average of 20.4% on blotter paper. On PDA the infestation of untreated seed samples were from 48.2 to 58.8% with an average of 52.52%, while the seeds treated with 2% Mercuric chloride solution, showed an infestation of 18.8 to 23.6% with an average of 20.87%.The fungi isolated from treated seeds were Fusarium moniliforme, Alternaria alternata, Mucor hiemalis, Chaetomium spp., Penicillium citrinum, Aspergillus niger, A. flavus, A .terreus and Nigrospora spp., F. moniliforme, A. alternata, M. hiemalis, Chaetomium spp., and A. niger were common in all samples while P. citrinum, A. flavus, A. terreus and Nigrospora spp., were only isolated from untreated seed.

¹Regional Agricultural Research Institute, Bahawalpur,Pakistan.

²Department of Plant Pathology, University of Arid Agriculture, Rawalpindi,Pakistan

³Greenbelt Project, Rawalpindi, Pakistan.

^*Corresponding author’s E-mail: drtmukhtar@uaar.edu.pk