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GENETIC VARIATION AMONG PHYTOPHTHORA INFESTANS (TOMATO BLIGHT) ISOLATES FROM WESTERN TURKEY REVEALED BY INTER SIMPLE SEQUENCE REPEAT (ISSR) AND RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS

NECIP TOSUN¹ ARZU YILDIRIM², HUSEYIN TURKUSAY¹ AND BAHATTIN TANYOLAC²

Abstract: Severe late blight disease epidemics in western Turkey during the 1997 tomato growing season prompted an investigation into the genotypes and mating types of Phytophthora infestans populations. A western Turkey-wide survey was conducted from 1999 to 2000 in which isolates were collected from 25 different fields. Twenty-five isolates were amplified with 21 RAPD and 18 ISSR primers. The isolates were analyzed for mating type. Genetic variation ranged between 0.002 (M. Kemalpasa (5) and M. Kemalpasa (12)) and 0.290 (Uluabat (46) and Uluabat (39)). Cluster analysis formed two main groups, one of which contained US-1 (A1 mating type) and the other US-8 (A2 mating type). Analysis of RAPD marker data indicated that in clade I, US-1 and Ulubat (46) were closest, while US-8 and Y. Sehir (26) were closest in clade II. This study shows that the A2 mating type of Phytophthora infestans exists in Turkey.

¹Department of Plant Pathology, Agriculture College of Ege University, 35100 Bornova-Izmir, Turkey

²Department of Bioengineering, Ege University 35100 Bornova-Izmir, Turkey

Corresponding Author's E-mail: bahattin.tanyolac@ege.edu.tr