Pak. J. Bot., 39(2): 623-627, 2007.

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			Updated: 09-07-09
	TECHNIQUE FOR IMPROVED PRODUCTION OF 3,4 DIHYDROXY PHENYL L-ALANINE BY ASPERGILLUS ORYZAE SIKANDER ALI AND IKRAM-UL-HAQ Abstract: In the present study, mould mycelium of Aspergillus oryzae was used for biochemical conversion of L-tyrosine to L-DOPA because tyrosinase is an intracellular enzyme. The mutant Aspergillus oryzae IIB-6 was found to yield 3.72 fold higher production of L-DOPA than the parental strain. The comparison of kinetic parameters was also done which showed the greater ability of mutant to yield L-DOPA. When cultures grown on various incubation periods, were monitored for Qp, Qs and qp, there was significant enhancement (p<0.0025–0.005) in these variables by mutant strain of A. oryzae IIB-2 over GCB-47 on all the rates. Institute of Industrial Biotechnology, Government College University Lahore, Pakistan.

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