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DNA MARKER FOR LEAF RUST DISEASE IN WHEAT

TANWIR AHMAD MALIK, AZHAR IQBAL, MUHAMMAD ASLAM CHOWDHRY, MUHAMMAD KASHIF AND SAJID-UR-RAHMAN

Abstract: Leaf (Brown) rust is major disease of wheat in Pakistan as well as in other countries. The objective of the study was to find DNA marker(s) linked to leaf rust resistance trait for use in tailoring disease resistant wheat cultivar. PCR technique and bulked segregant analysis was used to identify DNA marker linked to leaf rust resistant gene in F₂ segregating population. The F₂ population derived from the cross involving leaf rust susceptible, SK-7 and resistant parent, PBG-8881 with Lr29 resistant gene was scored for disease resistance. The F₂ population segregated into resistant and susceptible plants in a ratio of 3:1 showing monogenic inheritance. The primer 60-5 amplified a polymorphic molecules of 1100 base pairs from the genomic DNA of resistant plant. This DNA molecule can be used as DNA marker to identify leaf rust resistant plants in a breeding programme for developing rust resistant wheat cultivars.
 

Department of Plant Breeding & Genetics, University of Agriculture, Faisalabad, Pakistan.