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SONICATION ASSISTED AGROBACTERIUM MEDIATED TRANSFORMATION (SAAT): AN ALTERNATIVE METHOD FOR COTTON TRANSFORMATION

SYED SARFRAZ HUSSAIN*, TAYYAB HUSNAIN¹ AND S. RIAZUDDIN¹

Abstract: We report here a new procedure for cotton transformation based on cavitations caused by sonication which results in thousands of micro wounds on and below the surface of plant tissue and allow Agrobacterium to travel deeper and completely throughout the tissue. This wounding fashion increases the probability of infecting plant cells lying deeper in tissue. Many parameters were optimized for the enhancement of GUS transient expression in cotton using mature embryos as explant. GUS was first detected 24h following incubation of the explants and by 48h, GUS expression was very intense which served as a useful indicator of successful transformation of the cotton explant following sonication assisted Agrobacterium mediated transformation (SAAT) procedure. The study also showed the competitive advantage of this procedure over other transformation procedures being routinely used.
 

Department of Biosciences, COMSATS Institute of Information Technology, Plot # 30, H-8/1, Islamabad, Pakistan
¹ National Centre of Excellence in Molecular Biology, University of the Punjab, 87-West Canal Bank Road Thokar Niaz Baig Lahore-53700 Pakistan
Corresponding author E-Mail: syedsarfraz_2000@yahoo.com