Pak. J. Bot., 38(2): 407-415, 2006. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
HAPLOID PRODUCTION VARIATION IN SEVERAL DURUM WHEAT CULTIVARS AND THEIR SYNTHETIC HEXAPLOID DERIVATIVES A. MUJEEB-KAZI, J. AHMED*, A. GUL AND J. I. MIRZA
Abstract:
Bread wheat haploids are being routinely produced by the sexual wheat/maize
crossing protocol with perfect outputs across all its genotypes. A similar
success however, has not been achieved for durum wheat cultivars. We
evaluated 44 elite durum (Triticum turgidum L; 2n = 4x = 28,
AABB) high yielding, widely adapted cultivars for their ability to generate
haploids (n = 2x = 14, AB) when crossed by Zea mays (2n = 2x
= 20). This gave a range from 0% to 12.3% across the 44 genotypes. Synthetic
hexaploid (SH's) wheats derived from each of the 44 cultivars x Aegilops
tauschii accessions gave a haploid frequency range from 2.5 to 42.6%
providing unequivocal evidence that the D genome of Ae. tauschii
significantly influenced haploid production efficiency in SH wheats.
Durum cultivars amenable to haploidy induction are good candidates for
molecular studies and gene transfer programs where doubled haploids
have a distinct advantage. National Agricultural Research Center, Islamabad, Pakistan. Agricultural Biotechnology Research Institute, AARI, Faisalabad. |
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