Pak. J. Bot., 38(2): 393-406, 2006. | Back to Contents | ||||
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Updated: 09-07-09 | ||||
A SIMPLIFIED AND EFFECTIVE PROTOCOL FOR PRODUCTION OF BREAD WHEAT HAPLOIDS (n = 3x = 21, ABD) WITH SOME APPLICATION AREAS IN WHEAT IMPROVEMENT A. MUJEEB-KAZI, A. GUL, J. AHMED* AND J. I. MIRZA
Abstract:
Somaclonal variation, aneuploidy, and genotypic specificity are major
limitations of anther culture and bread wheat x Hordeum bulbosum
crosses for producing haploids (n=3x=21, ABD). Sexual combinations of
wheat x Zea mays have since emerged as an alternate and significant
procedure as wheat genotypic specificity does not exist. This procedure
has been refined and simplified over the last decade. It is in extensive
use in wheat breeding, cytogenetics, genetics, wide crosses, genetic
transformation and molecular mapping. Described here is this simplified
wheat x maize haploid production protocol that is 100% effective across
all bread wheat cultivars, generating data means of 25% for embryo excision,
90 to 95% for plantlet regeneration and between 95 to 100% for doubled
haploid (2n=6x=42, AABBDD) outputs. Simplification steps that enhance
efficiency involve hot water emasculation on detached tillers, bud-pollination,
elimination of the use of several exogenous chemicals between post-pollination
and embryo rescue with no cold shock given to the plated embryos; thus
making the product costs significantly competitive and economical.
National Agricultural Research Center, Islamabad, Pakistan. |
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